Fig. 3

S2Lb is a major determinant of the H3K4me3 landscape. a Immunoblot analysis of chromatin extracts from rosette leaves of wild-type, s2la-1, s2lb-2, s2la-1 s2lb-2, and complemented s2lb-2/S2Lb::S2Lb-GFP plant lines. Histone H3 methylation was detected using the indicated antibodies. Decreasing quantities of chromatin extracts from wild-type plants are shown for comparison, and detection of total histone H3 is used as a loading control. Quantification of H3-Lys4 methylation signals in S2L loss-of-function plants is given relative to their corresponding histone H3 levels. For each histone mark, levels in wild-type plants are arbitrarily set to 1. b ChIP-seq identification of the genes marked by H3K4me3 in WT, s2la-1, s2lb-2, and s2la-1 s2lb-2 seedlings in two independent biological replicates. The corresponding gene IDs are listed in Additional file 3: Table S2. c H3K4me3 enrichment over the genes marked in WT plants (N = 17,831). Genes are equally ranked from top to bottom in each plant line according to the H3K4me3 median enrichment in the WT. d H3K4me3 median enrichment over the genes marked in WT plants (N = 17,831). e Genome browser view of H3K4me3 enrichment over a representative region of chromosome 4. At4g16380 is shown as an example of a gene called by MACS2 as marked by H3K4me3 in WT and s2la-1 but not in s2lb-2 and s2la-1 s2lb-2 mutant lines. Each track represents the average data of two independent biological replicates. All tracks are equally scaled