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Fig. 2 | Genome Biology

Fig. 2

From: Arabidopsis S2Lb links AtCOMPASS-like and SDG2 activity in H3K4me3 independently from histone H2B monoubiquitination

Fig. 2

Growth defects of s2l mutant plants at the reproductive and vegetative stages. a Representative phenotypes of 4-week-old adult plants (left panel) and of dissected leaves (right panel) from plants grown under short-day conditions. Scale bar, 1 cm. b Rosette diameter at bolting of plants grown under-short (SD) or long-day (LD) photoperiods. Error bars indicate SD (n > 12). c Representative root phenotypes of 11-day-old seedlings. Scale bar, 0.5 cm. d Primary root length from 3 to 11 dpi. Error bars indicate SD from two biological replicates. e Seedling lateral root number at 11 dpi. Error bars indicate SD from two biological replicates. f Mean silique length and g number of seeds (or ovules) per silique in selfed wild-type and s2la/b mutant plants prior to seed desiccation. Black bars represent the mean number of undeveloped seeds per silique. Error bars indicate SD (n > 12 siliques each, from three individuals). h Enlarged view on a representative silique for each genotype. Arrows indicate undeveloped seeds/ovules. i Dormancy defects induced by S2Lb loss-of-function. The graph shows the percentage of freshly harvested germinating seeds from wild type, s2la, s2lb, and double s2las2lb mutant plants at 15 °C (vigor test, left graph) and 25 °C (dormancy test, right graph) in darkness. Error bars indicate SD between three biological replicates using two independent seed propagation sets (n = 50 seeds each). j RT-qPCR analysis of DOG1 transcripts level in seeds of wild-type and s2lb mutant plants upon imbibition. Total RNAs were extracted at the indicated time points. DOG1 expression level relative to housekeeping genes is given as the mean of three independent biological replicates. k Flowering time phenotype analyzed as the number of leaves at bolting under SD or LD conditions. Error bars indicate SD (n > 12). The letters indicate significant differences among sample means in ANOVA analysis followed by Tukey’s range test (p < 0.05)

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