Fig. 1

Profiling of circRNAs in the DLPFCs of 589 individuals. a The analysis pipeline to identify and quantify high-quality circRNAs. b A schematic diagram for calculating the circular ratio. c The stack diagram showing distributions of total gene counts and potential circular junction counts. The counts are log base 10 transformed on the y-axis. d The sample distribution for existing circRNAs in exonic, intronic, and intergenic regions. The black dashed line indicates the cumulative data fraction. e Gel analysis shows the target RT-PCR product bands. Red star points indicate the target band. f Two Sanger sequencing examples showed that RT-PCR products spanned the circular junction. Dashed line indicates the circular junction site. g Annotations of genomic regions mapping to inferred exonic circRNAs. CDS: coding sequence; lincRNA: long intergenic noncoding RNA; UTR: untranslated region