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Fig. 7 | Genome Biology

Fig. 7

From: VULCAN integrates ChIP-seq with patient-derived co-expression networks to identify GRHL2 as a key co-regulator of ERa at enhancers in breast cancer

Fig. 7

Estrogen time course and Co-IP of GRHL2. Analysis by western blot of the GRHL2 showed no changes in the levels of GRHL2 at 45 min, 90 min, or 24 h after stimulation with estradiol in either MCF7 or T47D. Co-IP of ER (bait, red, Santa Cruz:sc-8002) identified GRHL2 (green, Atlas: HPA004820) as an interactor in estrogenic conditions (M = marker, I = input, FT = flow through, IP = immunoprecipitation). siRNA knockdown of GRHL2 in MCF7 (right) resulted in a loss of the ~ 75-kDa band

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Genome Biology

ISSN: 1474-760X

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