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Fig. 7 | Genome Biology

Fig. 7

From: The inherited variations of a p53-responsive enhancer in 13q12.12 confer lung cancer risk by attenuating TNFRSF19 expression

Fig. 7

Analysis of the impacts of deregulated TNFRSF19 expression in vivo and in vitro. a The expression levels of TNFRSF19 in 117 NSCLC and the paired para-cancer tissue samples were detected by RT-qPCR analyses. The horizontal line indicates the mean expression level. (**p < 0.01, unpaired Student’s t test). b The expression levels of TNFRSF19 in 107 NSCLC tissues from TCGA on July 8, 2014, and c 81.31% showed downregulation of TNFRSF19 expression. The paired sample t test was used to examine the differences in gene expression between the tumors and adjacent normal tissues. d In total, 110 NSCLC lung cancer samples showed that the TNFRSF19 expression was negatively correlated with tumor staging. The horizontal line indicates the mean expression level. (**p < 0.01, unpaired Student’s t test). e TNFRSF19 expression was negatively correlated with the tumor size of 110 NSCLC lung cancer samples. The horizontal line indicates the mean expression level. (*p < 0.05, unpaired Student’s t test). f Analysis of the expression of TNFRSF19 at different N stages of 108 NSCLC lung cancer samples. The horizontal line indicates the mean expression level. (**p < 0.01, unpaired Student’s t test). g Kaplan-Meier analysis revealed a positive correlation between TNFRSF19 and overall survival (OS) in 1145 NSCLC patients. h A549 lung cancer cells that usually express low level of TNFRSF19 were infected with the lentivirus vectors expressing TNFRSF19 or the empty and were subsequently incubated in soft agar for colony formation (left and meddle panels). The colony numbers were statistically analyzed (right panel). The results showed that the introduction of TNFRSF19 into A549 cells significantly suppressed the colony formation. (n = 3 per group; error bars are SD; **p < 0.01, unpaired Student’s t test). i Transwell assays were used to evaluate the effect of the increase in TNFRSF19 on the invasive ability of the A549 cells (left and middle panels). The statistical analysis showed that the introduction of TNFRSF19 into the A549 cells significantly inhibited the cell invasion. (n = 3 per group; error bars are SD; **p < 0.01, unpaired Student’s t test)

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