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Fig. 6 | Genome Biology

Fig. 6

From: Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification

Fig. 6

Effect of instrument size bias on ATAC-Seq data. a Average insert size for 6 ATAC-Seq libraries sequenced on the HiSeq or NextSeq. b Percentage of reads at a subsampled depth of 20 million reads per sample classified as non-, mono-, di-, and tri-nucleosomal. n = 6 libraries. ***denotes p < 0.01 using a t-test. n.s. denotes no significant difference. c Distribution of mapped reads at the Fgfr4 locus. IGV plots of mapped reads for each sample, subsampled to a depth of 20 million reads, and either directly mapped (“All reads”) or split into the non-nucleosomal (“Non-nucl.”) subset and mapped. MACS peak calls for PAX3-responsive sites for HiSeq (top) and NextSeq (bottom) are below each set of mapped reads

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