Fig. 5From: Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantificationInstrument size biases affect genotyping marker observations in RAD-Seq data. a Average read counts for 11 RAD-Seq samples sequenced on the HiSeq or NextSeq. b Number of markers observed in filtered VCF file for the 11 RAD-Seq libraries. c Number of loci observed in filtered VCF file for the 11 RAD-Seq libraries. d Fraction of missing genotype calls for each sample in the unfiltered VCF file. e PCA plot generated using the unfiltered VCF file. f PCA plot using the filtered VCF file. HiSeq data points overlap with NextSeq data points in this plotBack to article page