Fig. 1

REcount enables accurate and precise measurements of plasmid pools. a Design of REcount constructs. A barcode-containing, Illumina adapter-flanked construct is liberated with a restriction enzyme (MlyI) digest and directly sequenced. b Accuracy and reproducibility of REcount. c Analogous measurements of the same plasmid pool shown in panel b using varying PCR cycle numbers. d Root mean squared deviation from expected values (5% per construct) when the plasmid pool is measured using REcount, and varying cycles of PCR amplification of either the barcode construct (BC) or another variable sequence in these plasmids (V4). e Pearson correlation heatmap comparing REcount measurements with droplet digital PCR data and with conventional PCR amplification of either the BC or V4 amplicons