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Fig. 4 | Genome Biology

Fig. 4

From: Translation of the circular RNA circβ-catenin promotes liver cancer cell growth through activation of the Wnt pathway

Fig. 4

Knockdown of circβ-catenin inhibited Wnt/β-catenin pathway. a After stable knockdown of circβ-catenin in PLC/PRF/5 cell line, gene ontology was conducted to analyze the pathways modulated by circβ-catenin. b Schematic illustration of the siRNAs targeting circβ-catenin or linear β-catenin mRNA. c The RNA levels of circβ-catenin and β-catenin mRNA were analyzed after knockdown of circβ-catenin or β-catenin mRNA in PLC/PRF/5 cells (n = 4). d The protein levels of β-catenin were analyzed after knockdown of circβ-catenin or β-catenin mRNA in PLC/PRF/5 cells. e The luciferase activities of TOPflash and FOPflash were measured after knockdown of circβ-catenin or β-catenin mRNA in PLC/PRF/5 cells (n = 4). f The expression of the β-catenin target gene was monitored by qRT-PCR in PLC/PRF/5 cells (n = 4) (*P < 0.05, **P < 0.01)

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Genome Biology

ISSN: 1474-760X

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