Fig. 3

Knockdown of circβ-catenin attenuated in vivo liver cancer cell growth and metastasis. a The liver cancer cells with stable knockdown of circβ-catenin were subcutaneously injected into nude mice, and the tumor tissues were collected at indicated time points (n = 5). b Tumor volume was measured and calculated (n = 5). c Tumor tissues were stained with Ki67 antibody, and the percentage of Ki67-positive cell was quantified by ImageJ. d Huh7 cells with stable knockdown of circβ-catenin were transplanted into the liver and the tumor size was observed after H&E staining (n = 5). The percentage of tumor area was quantified by ImageJ. e By using Huh7 cell line, lung metastasis model was established to examine the effect of circβ-catenin in modulating cancer metastasis (n = 5) (*P < 0.05, **P < 0.01)