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Fig. 1 | Genome Biology

Fig. 1

From: Translation of the circular RNA circβ-catenin promotes liver cancer cell growth through activation of the Wnt pathway

Fig. 1

Characterization of circβ-catenin in human cell lines and tissues. a The number of Wnt pathway-related circRNAs in human cell lines. b Genomic origin of Wnt pathway-related circRNAs in HepG2 cells. c The length distribution of Wnt pathway-related circRNAs in HepG2 cells. d Schematic illustration of the structure of circular RNA circβ-catenin. Sanger sequencing was conducted to confirm head-to-tail splicing. e Convergent and divergent primers were designed to detect linear and circular β-catenin RNAs, respectively. f Circβ-catenin was resistant to RNase-R treatment (n = 4). g Northern blotting was conducted to evaluate the RNA level of circular RNA and linear mRNA for β-catenin in PLC/PRF/5 cells. h The expression of circβ-catenin was assessed by qRT-PCR in the nuclear and cytoplasm fractions (n = 4). i The RNA levels of circβ-catenin and β-catenin mRNA were examined across 20 human tissues using qRT-PCR. j The correlation of circβ-catenin and β-catenin mRNA in 20 human tissues (**P < 0.01)

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