Fig. 8
From: DegNorm: normalization of generalized transcript degradation improves accuracy in RNA-seq analysis
Results for simulation I. a Box plot of estimated DI scores where conditions A and B stand for control and treatment samples, respectively. b Heatmap of DI scores of genes sorted in the ascending order of the average DI score of condition A (control), showing no between-condition clustered pattern. c–f Scatter plot of normalized read count vs. latent read count in log2 scale with diagonal line imposed for simulation I. Compared are results from proposed DegNorm pipeline and other methods including upper quartile (UQ), RUVr, and TIN. g, h ECDF plots of p value and ROC curves for simulation I under different normalization methods. All curves except TIN were well overlapping with each other, suggesting close performance in DE analysis