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Fig. 3 | Genome Biology

Fig. 3

From: Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses

Fig. 3

Deep sequencing of CRISPR-edited viruses in cassava transgenics. a Analysis of virus sequences from infected plants at 8 weeks post infection. Each horizontal line represents a 90-nt window for each individual virus sequence. Peaks represent edits and are scaled to the %mismatch value of each base pair (see “Methods” for calculation) in a pairwise global alignment with the reference virus sequence. The sgRNA target is indicated by a shaded red rectangle and a dark line represents the putative cut-site. b The number of instances of each substitution event in the AC2 protein detected in all the plant lines at 8 wpi. Green bars indicate amino acid substitutions in the sgRNA target region. c Distribution of ACMV-AC2 (H54Q) virus variant in different host plant lines. Percentage values represent the proportion of edited viruses in each line which contain the conserved CRISPR-induced mutation. d Alignment of some AC2 (H54Q) virus sequences with the sgRNA1 sequence

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