Fig. 4

Examination of candidate genes from QTL analysis RecQ-7 and RuvB. a Box plot comparison of the knockout RuvB lines with the control lines, defining CO frequency using collapsed linkage windows as per CO-Phenotype. b Box plot comparison of the knockout RecQ-7 lines with the control lines, defining CO/GC frequency using GC-Phenotype. c Box plot comparison of the knockout RecQ-7 lines with the control lines, defining CO frequency using CO-Phenotype. d Phylogenetic tree of identified genes across multiple species (Arabidopsis, rice and wheat) with sequence similarity to the RecQ helicase family, including our wheat candidate RecQ-7 gene for comparison. Bootstrap values ≥ 90% are shown as green dots on the branches