Fig. 5

BRB-seq performance with fragmented RNA samples. a Pearson correlation between log2 read counts of intact (RNA quality number (RQN) = 8.9 and 9.8 for T0 and T14 respectively) versus fragmented samples (after 1 or 2 min of fragmentation). b Quality evaluation of BRB-seq libraries prepared with fragmented RNA samples (1 or 2 min fragmentation) compared with the intact RNA counterparts. For the analysis, the libraries were downsampled to 1M single-end reads (see the “Methods” section). “Max” threshold thus comes from the 1M downsampled intact RNA sample when compared to itself, without downsampling. Legend: RQN, RNA quality number (maximum is 10)