Fig. 8

Lateral gene transfer introduction and subsequent evolution within the Hemiptera for mannosidase-encoding genes. a Species tree summary of evolutionary events. Stars represent the original LGT introduction and subsequent copy number gains (see legend). b Maximum likelihood phylogeny of mannosidase proteins, including bacterial sequences identified among the best GenBank blastp hits for Oncopeltus and Halyomorpha (accession numbers as indicated, and for “Other bacteria” are ACB22214.1, AEE17431.1, AEI12929.1, AEO43249.1, AFN74531.1, CDM56239.1, CUA67033.1, KOE98396.1, KPI24888.1, OAN41395.1, ODP26899.1, ODS11151.1, OON18663.1, PBD05534.1, SIR54690.1, WP096035621.1, YP001327394.1). All nodes have ≥ 50% support from 500 bootstrap replicates [167]. Triangles are shown to scale for branch length and number of clade members; branch length unit is substitutions per site. See also Additional file 1: Figure S2.6. c Manually curated protein sequence alignment for the N-terminal region only. Splice sites (“|” symbol) are shown, where one position is ancestral and present in all paralogs of a given species (magenta) and one position occurs in a subset of paralogs and is presumed to be younger (cyan, within the 5′ UTR in Halyomorpha). Residues highlighted in yellow are conserved between the two hemipteran species. The Oncopeltus paralog represented in the OGS as OFAS017153-RA is marked with an asterisk to indicate that this version of the gene model is incomplete and lacks the initial exon (gray text in the alignment). For clarity, only the final three digits of the Halyomorpha GenBank accessions are shown (full accessions: XP_014289XXX)