Fig. 3

Orthology comparisons and phylogenetic placement of Oncopeltus fasciatus among other Arthropoda. a Comparisons of protein-coding genes in 12 arthropod species, with the Hemiptera highlighted in red text. The bar chart shows the number of proteins per conservation level (see legend), based on OrthoDB orthology clustering analyses. To the left is a maximum likelihood phylogeny based on concatenation of 395 single-copy orthologs (all nodes have 100% support unless otherwise noted; branch length unit is substitutions per site). The inset pie chart shows the proportion of proteins per conservation level in Oncopeltus (Ofas). See also Additional file 1: Supplemental Note 6.1. b BUSCO-based analysis of Oncopeltus compared to other hemipterans for ortholog presence and copy number in both the assembly and OGS resources, using 4-letter species abbreviations (full names in a). c Proportion of Oncopeltus proteins that have expression and/or curation validation support per conservation level (same color legend as in a). Expression support is based on the life history stage data in Fig. 2b. Analyses are based on OGS v1.1