Fig. 2

Comparisons of the official gene set and transcriptomic resources for Oncopeltus fasciatus. a Area-proportional Venn diagram comparing the OGS v1.1 (OGS), a Trinity de novo transcriptome from the three post-embryonic RNA-seq samples (i5K) and the maternal and embryonic transcriptome from 454 data (“454”, [35]). Sample sizes and the fraction of each transcriptome represented in the OGS are indicated (for the 454 dataset, only transcripts with homology identification were considered). The unique fraction of each set is also specified (%). Dataset overlaps were determined by blastn (best hit only, e value < 10⁻⁹). b Venn diagram of gene model expression support across four life history samples. Values are numbers of gene models, with percentages also given for the largest subsets. Note that the “Embryo/Maternal” sample derives from 454 pyrosequencing data and therefore has a smaller data volume than the other, Illumina-based samples. c Summary of sex- and developmental stage-specific RNA-seq comparisons across hemipteroid species: Apis, Acyrthosiphon pisum; Clec, Cimex lectularius; Focc, Frankliniella occidentalis (thysanopteran outgroup); Ofas, Oncopeltus fasciatus; Pven, Pachypsylla venusta; n.d., not determined. For complete numerical details, see Additional file 1: Supplemental Note 2.4. Analyses are based on OGS v1.1