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Fig. 1 | Genome Biology

Fig. 1

From: Analysis of error profiles in deep next-generation sequencing data

Fig. 1

Potential error sources in next-generation sequencing workflow. a Illustration of the major steps of a typical next-generation sequencing workflow. Targeted deep sequencing is usually done by amplicon protocol or hybridization-capture protocol. Potential error sources are indicated by numbers. b Percentage of high-quality (Q30) bases by position in NGS read. This shows that the first and the last 5 bp have lower percentages of high-quality bases than do other positions. c Cumulative plot of NGS read quality distribution categorized by low-quality mapping (MAPQ < 55), potentially problematic alignment (“Methods”), and number of poor-quality bases in read (from ≥ 16 bp to 0 bp per read)

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