Fig. 5

Reporter lines for AT1G64660 confirm imprinted expression. Representative images of seeds derived after reciprocal crosses of the AT1G64660 reporter line (fusion with green fluorescent protein (GFP)) with wild-type (WT) plants. GFP fluorescence was detected in the seed coat when the AT1G64660 reporter was maternally inherited, but endosperm-specific expression was only detected when the AT1G64660 reporter was paternally inherited. Seeds at 2 DAP were used for imaging. Red stain is propidium iodide. Scale bars correspond to 50 μm