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Fig. 3 | Genome Biology

Fig. 3

From: Epigenetic signatures associated with imprinted paternally expressed genes in the Arabidopsis endosperm

Fig. 3

Paternally biased expression coincides with the combination of H3K27me3, H3K9me2, and CHG methylation in crosses Col × Ler (for reciprocal cross direction see Additional file 1: Figure S5). a Box plots show mean values of maternal to total reads for genes marked by one or several of the following modifications: CHG methylation in the central cell, difference of CHG methylation, H3K27me3, or H3K9me2 levels on maternal and paternal alleles in the endosperm. For each modification only genes present in the highest scoring category were included in the analysis (see Additional file 2: Table S1). Boxes show medians and the interquartile range; error bars show the full range excluding outliers. Widths of boxes are proportional to square-root of the number of genes in each category. Blue line indicates the expected ratio for biallelically expressed genes. b Paternal expression bias correlates with high levels of CHG methylation in the central cell and H3K27me3 and H3K9me2 on maternal alleles in the endosperm. Plotted are mean values of CHG methylation, H3K27me3, and H3K9me2 on maternal and paternal alleles for genes with deviating parental expression levels from the expected two maternal to one paternal ratio

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