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Fig. 5 | Genome Biology

Fig. 5

From: Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells

Fig. 5

Induction of A3G-mediated C>U mRNA editing by the inhibition of mitochondrial respiration. a Immunoblot showing the protein level of HIF-1α in whole cell lysates of HuT78 when subjected to normoxia (N) and hypoxia (H) in 96-well (W) and 6-W plates for 24 h. All lanes are part of the same gel. The dashed line represents the cropped region. The percentage of C>U RNA editing levels in TM7SF3 under these conditions is displayed below. b The percentage of C>U RNA editing in TM7SF3 when HuT78 cells are treated with myxothiazol (MXT), thapsigargin (Tg), Atpenin (AtA5), and hypoxia (H) for 24 h (day 1) or 42 h (day 2) (n = 3). c Immunoblot showing the protein level of HIF-1α in whole cell lysates of HuT78 when subjected to normoxia (N) with or without the mitochondrial inhibitors (MXT and AtA5) and hypoxia (H) for 1 or 2 days. d The percentage of C>U RNA editing in TM7SF3 when NK-92 cells are treated with myxothiazol (MXT), Atpenin (AtA5), and hypoxia (H) for 42 h (n = 3). See the “Methods” section for statistical analysis

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