Fig. 4

Knockdown of A3G reduces C>U RNA editing induced by cellular crowding in HuT78 cell line. a Immunoblot showing the protein levels of eIF-2α phosphorylated at Ser 51 in whole cell lysates of scramble CTRL and KD1a cells at various time points. Thapsigargin (Tg)-treated HuT78 cells is a positive control, and α-tubulin is used as a loading control. b A3G, A3F, and A3C gene expression in control and KD HuT78 cells by RT-qPCR. Gene expression measurements are normalized to that of β2-microglobulin. Expression of each gene is arbitrarily set to ten in control (CTRL) cell line to highlight specific targeting of A3G. c Immunoblot for A3G protein expression in whole cells lysates of WT (original wild-type cell line), scramble CTRL, and A3G KD HuT78 cells shows a reduction in A3G protein levels in KD1a and KD2. α-Tubulin is used as a loading control. d Graph representing the percentage site-specific C>U RNA editing level for TM7SF3, EIF3I, and RFX7 of scramble CTRL and KD HuT78 cells in normoxia with cellular crowding (n = 3, mean and SEM). N, normoxia with cellular crowding; H, hypoxia with cellular crowding; T0, normoxia without cellular crowding. See the “Methods” section for statistical analysis