Fig. 1

Cell-specific expression of A3G and the induction of RNA editing in NK cells. a Cell types that have the highest expression of A3G (probe:204205_at in Affymetrix HG-U133) in Primary Cell Atlas, a meta-analysis of publicly available 100+ microarray datasets, are shown (see Additional file 1; Figure S1 for other immune cells). b A3G gene expression in primary NK, CD4+ T, and CD8+ T cells by qPCR (n = 3 donors, T0 and hypoxia levels combined, mean and SEM shown). Gene expression measurements are normalized to that of β2-microglobulin. c Immunoblot showing the protein levels of eIF-2α phosphorylated at Ser 51 in whole cell lysates of NK cells at 0, 20, and 40 h under normoxia (N) or hypoxia (H). Thapsigargin (Tg)-treated NK cells are used as a positive control, and β-actin is used as a loading control. d Sanger sequence chromatogram traces of RT-PCR products of TM7SF3 in unstressed (uncrowded baseline, T0) and stressed (crowding in normoxia (N) or crowding in hypoxia (H)) NK cells. Edited C is highlighted in black. e Estimation of site-specific C>U RNA editing by Sanger sequencing of RT-PCR products for TM7SF3, RPL10A, and RFX7 in NK, CD4+ T, and CD8+ T cells subjected to crowding and hypoxia. (n = 3 donors, mean and SEM are shown). See the “Methods” section for statistical analysis