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Table 2 A small component of the reporting details for MAGs as proposed by the Genomic Standards Consortium include ranks of quality [13]

From: bin3C: exploiting Hi-C sequencing data to accurately resolve metagenome-assembled genomes

Rank

Assembly Quality Criteria

Additionally

Finished

Single, validated contiguous sequence per replicon without gaps or ambiguities, with consensus error rate or equivalent > Q50

 
 

Completeness and contamination (%)

 

High-quality draft

>90, <5

Presence of 23S, 16S, and 5S and ≥18 tRNAs.

Medium-quality draft

≥50, <10

 

Low-quality draft

<50, <10

 
  1. The “finished” rank is left to future advances, while lower ranks are achievable now by Hi-C-based genome binning methods. The additional criterion of rRNA genes makes the “high-quality” rank challenging to achieve with current methods
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Genome Biology

ISSN: 1474-760X

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