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Fig. 2 | Genome Biology

Fig. 2

From: Evolutionarily significant A-to-I RNA editing events originated through G-to-A mutations in primates

Fig. 2

Evaluation and validation of hgG and polyAG editing sites in rhesus macaque. a The enriched and depleted nucleotide sequences flanking the Alu-associated A-to-I RNA editing sites are shown for macaque-specific editing sites (hgG), A/G polymorphic editing sites (polyAG), and A/G heterozygote sites (Het A/G) as negative control, with the level of preference or depletion presented in height proportional to the scale. b The distributions of editing levels in six macaque tissues, estimated as the frequency of G-harboring reads in rRNA-depleted RNA-seq data, are shown for all Alu-associated A-to-I editing sites (All), macaque-specific editing sites (hgG) and A/G polymorphic editing sites (polyAG). As a negative control, the frequencies of G for A/G heterozygote sites were also estimated with RNA-seq data (Het A/G). c Scatter plot shows the frequency of the G allele in 54 randomly selected polyAG sites, as estimated by using 31 macaque animals with whole genome sequencing, and 82 animals with targeted DNA sequencing. d Two exemplary polyAG/hgG editing sites located on the A/G polymorphic sites in the macaque population and also genomically encoded as G in human were verified with Sanger sequencing. Two editing sites (indicated by arrow) were identified in cDNA samples from six tissues of the same macaque animal (100MGP-001). The focal editing sites are A/G polymorphic in a macaque population of six animals, including 100MGP-001 (“Methods”), and are genomically encoded as G in a human population of six healthy individuals (“Methods”)

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