Table 1 Genotype of all 19 pups born following TALEN injections
From: Gene editing of the multi-copy H2A.B gene and its importance for fertility
# | Pup ID | Genotype by Sanger sequencing | ||
|---|---|---|---|---|
H2Afb3 | Gm14920 | H2Afb2 | ||
1 | ♀ L74-1 | XX (wt) | XX (wt) | XX (wt) |
2 | ♀ L74-2 | XX (wt) | XX (wt) | XX (wt) |
3 | ♀ L74-3 | XX (wt) | XX (wt) | XX (wt) |
4 | ♂ L74-4 | XY (wt) | XY (wt) | XY (wt) |
5 | ♂ L74-5* | X ∆5 + 286 Y | X ∆15 Y | X ∆17 Y |
6 | ♀ L79-1 | X ∆10 X ∆10 | X ∆12 X ∆12 Chimera of b/∆/c | X ∆15 X ∆15 Chimera of c/∆/b |
7 | ♀ L89-1* | X ∆22 X ∆160 | X ∆5 X ∆17 Chimera c/∆/b) | X ∆5 X ∆5 |
8 | ♂ L89-2 | X ∆65 Y | X C/T, A/G Y | X∆42 Y Chimera b/∆/c) |
9 | ♂ L89-3 | X ∆26 Y Chimera c/∆/a) | X ∆10 Y Chimera b/∆/c) | X ∆10 Y Chimera b/∆/c) |
10 | ♀ L90-1 | XX (wt) | XX (wt) | XX (wt) |
11 | ♀ L90-2 | XX (wt) | XX (wt) | XX (wt) |
12 | ♀ L90-3* | X ∆28 X ∆5 | X ∆10 X ∆10 | X ∆64 X ∆64 |
13 | ♀ L90-4 | X ∆15 X ∆10 | No successful PCR amplification | No successful PCR amplification. |
14 | ♀ L90-5 | XX (wt) | XX (wt) | XX (wt) |
15 | ♀ L90-6 | X ∆17 X ∆17 Chimera c/∆/a) | X ∆5 X ∆5 Chimera b/∆/c) | X ∆5 X ∆5 Chimera c/b/∆/c) |
16 | ♀ L90-7 | XX (wt) | XX (wt) | XX (wt) |
17 | ♂ L90-8 | X ∆16 Y | X ∆17 Y Chimera c/∆/c/b) | No successful PCR amplification. |
18 | ♂ L90-9 | XY (wt) | XY (wt) | XY (wt) |
19 | ♂ L90-10 | XY (wt) | XY (wt) | XY (wt) |