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Table 1 Genotype of all 19 pups born following TALEN injections

From: Gene editing of the multi-copy H2A.B gene and its importance for fertility

# Pup ID Genotype by Sanger sequencing
H2Afb3 Gm14920 H2Afb2
1 ♀ L74-1 XX (wt) XX (wt) XX (wt)
2 ♀ L74-2 XX (wt) XX (wt) XX (wt)
3 ♀ L74-3 XX (wt) XX (wt) XX (wt)
4 ♂ L74-4 XY (wt) XY (wt) XY (wt)
5 ♂ L74-5* X ∆5 + 286 Y X ∆15 Y X ∆17 Y
6 ♀ L79-1 X ∆10 X ∆10 X ∆12 X ∆12
Chimera of b/∆/c
X ∆15 X ∆15
Chimera of c/∆/b
7 ♀ L89-1* X ∆22 X ∆160 X ∆5 X ∆17
Chimera c/∆/b)
X ∆5 X ∆5
8 ♂ L89-2 X ∆65 Y X C/T, A/G Y X∆42 Y
Chimera b/∆/c)
9 ♂ L89-3 X ∆26 Y
Chimera c/∆/a)
X ∆10 Y
Chimera b/∆/c)
X ∆10 Y
Chimera b/∆/c)
10 ♀ L90-1 XX (wt) XX (wt) XX (wt)
11 ♀ L90-2 XX (wt) XX (wt) XX (wt)
12 ♀ L90-3* X ∆28 X ∆5 X ∆10 X ∆10 X ∆64 X ∆64
13 ♀ L90-4 X ∆15 X ∆10 No successful PCR amplification No successful PCR amplification.
14 ♀ L90-5 XX (wt) XX (wt) XX (wt)
15 ♀ L90-6 X ∆17 X ∆17
Chimera c/∆/a)
X ∆5 X ∆5
Chimera b/∆/c)
X ∆5 X ∆5
Chimera c/b/∆/c)
16 ♀ L90-7 XX (wt) XX (wt) XX (wt)
17 ♂ L90-8 X ∆16 Y X ∆17 Y
Chimera c/∆/c/b)
No successful PCR amplification.
18 ♂ L90-9 XY (wt) XY (wt) XY (wt)
19 ♂ L90-10 XY (wt) XY (wt) XY (wt)
  1. wt, wild type offspring; ∆n, deletion of n nucleotides; +n, insertion of n nucleotides. *Denotes mice that were used as founders to establish H2A.B.3 KO colonies. The nine Talen induced mutations are italicized