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Fig. 1 | Genome Biology

Fig. 1

From: Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Fig. 1

Comparing the effects of Ddx19B or Dicer1 knockdown by Trim-Away or morpholinos in zebrafish embryos. Zebrafish embryos were co-injected with TRIM21 and antibodies (anti-Ddx19B antibody, anti-Dicer1 antibody, or control IgG) or injected with morpholinos (ddx19b-MO, Dicer1-MO, or Con-MO) at 0 hpf. a The level of Ddx19B or Dicer1 was determined by western blotting at the indicated time points. b Lateral view of eye size at the indicated time points. c Lateral view of the body axis at 2 dpf. d Eye diameter in embryos injected with different doses of TRIM21/anti-Ddx19B antibody or TRIM21/IgG was measured at the indicated time points; zebrafish numbers are indicated in brackets. e The level of miR-26a and let-7a was determined at the indicated time points. f Lateral view of embryos at the indicated time points. The data in d and e are shown as the mean ± SD of three independent experiments, *P ≤ 0.05, **P ≤ 0.01, NS indicates P > 0.05

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Genome Biology

ISSN: 1474-760X

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