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Fig. 9 | Genome Biology

Fig. 9

From: Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens

Fig. 9

Effects of genetic variation on guide on-target activity. In the Avana library, 472 guides target protospacer sequences overlapping SNPs assayed by the Affymetrix SNP 6/0 array. a Distribution of correlations between guide log-fold changes and SNP genotype (genotype/LFC correlations) for the 472 affected guides (red line). A null distribution was obtained from permuting SNP genotypes B=100 times (gray line). b Genotype/LFC correlations as a function of the SNP location within the protospacer sequence. c Log-fold changes for a guide targeting EIF2B3 and overlapping the SNP rs11556200. The genotype GG represents the reference allele found in the GRCh38 genome assembly. d Log-fold changes for all guides targeting EIF2B3. e CERES score for EIF2B3 as a function of the SNP rs11556200 genotype. f Log-fold change for a guide targeting OAS1 and overlapping the SNP rs1131454. The genotype GG represents the reference allele found in the GRCh38 genome assembly

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