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Fig. 6 | Genome Biology

Fig. 6

From: Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens

Fig. 6

Melanoma cell lines with high expression of SOX10 are not sensitive to SOX9 knockdown. a Left panel: log-fold change for clean guide A3 targeting SOX9 as a function of SOX9 expression. Right panel: log-fold change for clean guide B1 targeting SOX10 as a function of SOX10 expression. Two melanoma cell lines were selected for SOX9 and SOX10 knockdowns validation: Malem-3M and UACC-62 (in red). One colorectal carcinoma (LS1034) was selected to measure SOX9 siRNAs knockdown efficiency. b Cell viability of two melanoma cell lines after transfection with small interfering RNAs (siRNAs), relative to the non-targeting control (siNTC) treatment. c Relative SOX10 mRNA expression levels, detected by qPCR, after transfection of the indicated siRNAs, for two melanoma cell lines. Expression levels are normalized to GAPDH mRNA detected in the same sample, and then expressed as a fold change with respect to the siNTC treatment. d Relative SOX9 mRNA expression levels, detected by qPCR, after transfection of the indicated siRNAs, for the colorectal carcinoma cell line LS1034

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