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Fig. 3 | Genome Biology

Fig. 3

From: Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens

Fig. 3

Analysis of the genetic knockout additivity constraints for TMED7, TICAM2 and the TMED7-TICAM2 readthrough. a Design for guides targeting TMED7, TICAM2 and the readthrough TMED7-TICAM2 in the Avana library. b Cell line-specific gene score solutions for an additive model using the Avana guides targeting TMED7, TICAM2, and the readthrough TMED7-TICAM2. yi denotes the LFC for guide i and βFusion denotes the gene score for the readthrough TMED7-TICAM2. Explicit solutions are derived using ordinary least squares (OLS). c Pairwise scatterplots for the CERES scores for TMED7, TICAM2 and the readthrough TMED7-TICAM2. d Pairwise scatterplots for the DEMETER scores (RNAi) for TMED7, TICAM2, and the readthrough TMED7-TICAM2

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