Fig. 5

Regulation of lincRNAs by miRNAs. a Relative GFP expression level of linc-60 in N2 worms with or without the overexpression of mir-5550 (n = 20). b Relative GFP expression level of linc-109 in N2 worms with or without the overexpression of mir-355 (n = 20). c Relative GFP expression level of linc-109 in N2 worms with or without the overexpression of mir-254 (n = 20). d Relative GFP expression level of linc-109 in N2 worms with or without the overexpression of mir-4934 (n = 20). Constructs with mutations in the miRNA binding site of lincRNA were used as negative controls, and the positions of mutations are represented by red crosses. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001; Student’s t test. Data are the means ± SD. Images shown are representative of the control and experimental groups. Scale bar, 20 μm