TY - JOUR AU - Yuan, Jinzhou AU - Sheng, Jenny AU - Sims, Peter A. PY - 2018 DA - 2018/12/24 TI - SCOPE-Seq: a scalable technology for linking live cell imaging and single-cell RNA sequencing JO - Genome Biology SP - 227 VL - 19 IS - 1 AB - Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We combine microfluidics with optically decodable beads and show that phenotypic analysis of living cells can be linked to single-cell sequencing. As a proof-of-concept, we demonstrate the accuracy and scalability of our tool called Single Cell Optical Phenotyping and Expression sequencing (SCOPE-Seq) to combine live cell imaging with single-cell RNA sequencing. SN - 1474-760X UR - https://doi.org/10.1186/s13059-018-1607-x DO - 10.1186/s13059-018-1607-x ID - Yuan2018 ER -