Fig. 2

Concordance of TADs identified using different normalization strategies and resolutions. a Concordance between TAD partitions obtained with each TAD caller from ICE- and LGF-normalized matrices at five different resolutions (10, 50, 100, 250 kb) was assessed using the Measure of Concordance (MoC). MoC varies from 0 (absence of concordance, white) to 1 (full concordance, dark red). TAD callers are ranked based on the average of the MoC values across all resolutions (from highest to lowest). TAD callers that did not successfully identified TADs at a given resolution (gray boxes) were scored as 0 for the purpose of ranking by average MoC. b Concordance between TAD partitions obtained at different resolutions was assessed in a pairwise manner (e.g., 10 kb vs. 50 kb, 10 kb vs. 100 kb, etc.; results for the ICE data only are shown here) using the MoC. MoC varies from 0 (absence of concordance, white) to 1 (full concordance, dark red). TAD callers are ranked based on the average of the MoC values across all comparisons (from highest to lowest), and resolution comparisons are ordered according to the fold difference between matrix resolutions. TAD callers that did not successfully identified TADs at a given resolution (gray boxes) were scored as 0 for the purpose of ranking by average MoC. c Concordance across normalizations (average MoC obtained by comparing ICE and LGF partitions at different resolutions) versus concordance across resolutions (average MoC obtained by comparing Hi-C matrix of different resolutions for a fixed normalization. The dashed line indicates the linear fit