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Fig. 3 | Genome Biology

Fig. 3

From: A novel FLI1 exonic circular RNA promotes metastasis in breast cancer by coordinately regulating TET1 and DNMT1

Fig. 3

FLI1 circular RNA promotes invasion of breast cancer cells. a Ectopic expression of FECR1 in MDA-MB231 breast cancer cells. The FECR1 expression cassette is composed of FLI1 exons 4-2-3 and the intron fragments containing the back-splicing elements. pCMV, CMV promoter; pEF1a, EF1a promoter. DsRed fluorescent marker was used to track the transfection. b RT-PCR of FECR1. After stable transfection, cells were collected and FECR1 was amplified by PCR. PBS and vector, control groups. c Quantitation of FECR1 by qPCR. **p < 0.01 as compared with PBS and vector controls. d FECR1 promotes cell invasion. Cells that crossed through the collagen-coated membrane of the transwell were stained and photographed. e Quantitation of invading cells. All data shown are mean ± SEM from three independent experiments. **p < 0.01 as compared with PBS and vector control groups

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