Table 2 Number of predicted near-cognate sites in the human genome for the three dual target sites (DTS3, DTS7 and DTS8) analyzed in this study. These potential off-target sites differ from the on-target site by six or fewer mismatches, as listed on the left, and include the functional or semi-functional PAMs shown at the top
From: NmeCas9 is an intrinsically high-fidelity genome-editing platform
Number of mismatches | SpyCas9 sites (NGG, NGA, NAG PAMs) | NmeCas9 sites (N4GATT, N4GCTT, N4GTTT, N4GACT, N4GATA, N4GTCT, N4GACA PAMs) | NmeCas9 sites (N5GATT, N5GCTT, N5GTTT, N5GACT, N5GATA, N5GTCT, N5GACA “slipped” PAMs) | ||||||
|---|---|---|---|---|---|---|---|---|---|
DTS3 | DTS7 | DTS8 | DTS3 | DTS7 | DTS8 | DTS3 | DTS7 | DTS8 | |
1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
3 | 45 | 52 | 60 | 0 | 0 | 0 | 0 | 0 | 0 |
4 | 680 | 500 | 772 | 0 | 2 | 0 | 0 | 2 | 1 |
5 | 6691 | 4116 | 7325 | 4 | 5 | 25 | 0 | 4 | 12 |
6 | 45,897 | 26,474 | 52,547 | 17 | 61 | 129 | 24 | 62 | 124 |
Total | 53,317 | 31,144 | 60,706 | 21 | 68 | 154 | 24 | 68 | 137 |