Fig. 2
From: Linking single-cell measurements of mass, growth rate, and gene expression
Linked biophysical and gene expression measurements of single L1210 and FL5.12 cells. a Plot of mass accumulation rate versus buoyant mass for single L1210 cells (top, n = 234) and single FL5.12 cells (bottom, n = 296) measured in the sSMR. Kernel density plots are included on both axes. b Heat maps showing the relative expression of various cell cycle-related genes for subsets of the L1210 (top, n = 85) and FL5.12 (bottom, n = 124) cells depicted in (a) that were captured downstream for scRNA-seq. Cells are ordered by buoyant mass (bar plots above heat maps). Entries are colored by expression z-score. As a demonstration, the heat map includes genes with expression levels that showed a significant correlation with buoyant mass from the chromosome segregation (black bar, n = 58 and n = 31 for the L1210 and FL5.12, respectively) and DNA replication (gray bar, n = 11 and n = 8 for the L1210 and FL5.12, respectively) gene ontology subsets (FDR < 0.05, Additional file 1: Figure S4, Additional file 3: Table S2, Additional file 4: Table S3, Methods)