Fig. 2

Tetrahymena 6mA sites and nucleosome positions are anti-correlated. a Nucleosome positioning around TSS. The centers of nucleosomes are predicted and aligned to the 2 kbp region of each TSS. The counts of nucleosomes in each position are normalized to the total counts in the entire region. Two biological replicates were compared. b Anti-correlation pattern of 6mA and nucleosome at the downstream regions of TSS. c Nucleosome positioning around individual 6mA sites. The nucleosome occupancies around each 6mA site were accumulated and plotted. d Total 6mA levels of nucleosome-protected genomic DNA versus input DNA and unprotected regions. Error bars indicate mean ± s.d. of three technical replicates, each measured in duplicates. **p < 0.01 by Student’s t test. e Illustration of the anti-correlation distribution model. Purple cylinders wrapped with yellow curves represent nucleosomes and DNA. Red circles represent 6mA modifications on DNA