Fig. 1

Identification of 6mA in the genome of Tetrahymena. a Genomic components of genome-wide 6mA distribution. The enrichment score was calculated as the proportion of 6mA peaks dividing the proportion of the attributed genomic component occupying the entire genome. b 6mA profile revealed by 6mA-IP-seq. 6mA peaks were identified by comparing reads from IP to input and aligned to the flanking 2 kbp region of TSS. Two biological replicates were performed. c 6mA profile revealed by 6mA-CLIP-exo. Two biological replicates were performed. Peaks were aligned to TSS and accumulative distribution was depicted similarly to b. d 6mA sites revealed by 6mA-RE-seq at single-base resolution. Parallel experiments were performed on the same sample by using three different methylation-sensitive restriction enzymes (DpnI, DpnII, and CviAII). The accumulative individual 6mA sites were aligned to TSS region and depicted similarly to b