Fig. 2
From: DNA methylation and de-methylation using hybrid site-targeting proteins
Current strategies of targeted CpG methylation. a Main strategies of zinc-finger proteins (ZFP) or transcription activator-like effector (TALE)-associated hybrid proteins. Methyltransferase (MTase) can be fused to a ZFP or TALE anchoring platform with or without a linker. Split MTase can be fused to a ZFP or TALE pair to improve binding specificity. b Main strategies for the use of CRISPR-based hybrid proteins. MTase can be fused to dCas9 with or without a linker. Chimeric MTase such as DNMT3A–DNMT3L has been applied to increase methylation. Split MTase (M.SssI) can decrease off-target effects. MTase multimerization approaches (such as dCas9–SunTag) enhance activity for long-range methylation editing