Fig. 5

6mA level and landscape are altered in the Phytophthora DAMT3 mutant. a The 6mA levels of DAMT mutants were quantified by UPLC-ESI-MS/MS. WT is P. sojae wild-type strain P6497. psdamt1 (T21), psdamt2 (T52), and psdamt3 (T9) are representative lines from each of the DAMT gene knockout mutants generated by CRISPR/Cas9. **Significant differences (P < 0.01, Student’s t test). b A total of 890 (29.4%) 6mA peaks are lost in psdamt3. c Methylation level of methylated DNA elements (n = 480) and LTR elements (n = 867) are reduced in psdamt3. P values are calculated with the two-sample Kolmogorov-Smirnov test. d The average 6mA level of methylated genes (n = 1343) is reduced in the psdamt3 mutant. e Snapshot of 6mA deposition in the psdamt3 mutant demonstrates that 6mA reduced in representative genomic segment. Here, a methylation peak in Ps_116863 gene locus is used as an unaffected control