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Table 2 Assembly statistics from original NGS genome to RACA assembly and combined RACA and FISH assembly

From: Chromosome-level assembly reveals extensive rearrangement in saker falcon and budgerigar, but not ostrich, genomes

Original assembly

 Stats

Budgie

Ostrich

Saker falcon

  No. scaffolds longer 10 kbp

1138

1179

731

  Total length (Gbp)

1.08

1.22

1.17

  N50 (Mbp)

11.41

3.64

4.16

Default RACA assembly

 Stats

Budgerigar PCFs

Ostrich PCFs

Saker falcon PCFs

  No. PCFs

84

100

95

  Total length (Gbp)

1.04

1.17

1.14

  N50 (Mbp)

46.54

37.95

39.38

  No. chimeric scaffolds

80 (31%)

58 (10%)

50 (10%)

  No. used scaffolds

254

588

458

  % original assembly

96.29

95.90

97.26

RACA + PCR assembly

 Stats

Budgerigar PCFs

Ostrich PCFs

Saker falcon PCFs

  No. PCFs

95

136

103

  Total length (Gbp)

1.04

1.17

1.14

  N50 (Mbp)

37.96

28.09

22.28

  No. chimeric scaffolds

55 (21%)

31 (5%)

25 (5%)

  No. used scaffolds

254

588

458

  % original assembly

96.29

96.02

97.26

RACA + FISH assembly

 Stats

Budgerigar chromosomes

Ostrich chromosomes

Saker falcon chromosomes

  No. PCFs placed

46

53

64

  No. PCFs oriented

28

37

37

  Disagreements RACA-FISH

4

0

0

  Length placed (bp)

1,013,720,408

969,537,146

1,055,312,481

  Length oriented (bp)

844,433,024

869,521,333

790,725,803

  % original assembly placed

93.56

79.45

90.12

  % original assembly oriented

77.93

71.26

67.52