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Table 2 Assembly statistics from original NGS genome to RACA assembly and combined RACA and FISH assembly

From: Chromosome-level assembly reveals extensive rearrangement in saker falcon and budgerigar, but not ostrich, genomes

Original assembly
 Stats Budgie Ostrich Saker falcon
  No. scaffolds longer 10 kbp 1138 1179 731
  Total length (Gbp) 1.08 1.22 1.17
  N50 (Mbp) 11.41 3.64 4.16
Default RACA assembly
 Stats Budgerigar PCFs Ostrich PCFs Saker falcon PCFs
  No. PCFs 84 100 95
  Total length (Gbp) 1.04 1.17 1.14
  N50 (Mbp) 46.54 37.95 39.38
  No. chimeric scaffolds 80 (31%) 58 (10%) 50 (10%)
  No. used scaffolds 254 588 458
  % original assembly 96.29 95.90 97.26
RACA + PCR assembly
 Stats Budgerigar PCFs Ostrich PCFs Saker falcon PCFs
  No. PCFs 95 136 103
  Total length (Gbp) 1.04 1.17 1.14
  N50 (Mbp) 37.96 28.09 22.28
  No. chimeric scaffolds 55 (21%) 31 (5%) 25 (5%)
  No. used scaffolds 254 588 458
  % original assembly 96.29 96.02 97.26
RACA + FISH assembly
 Stats Budgerigar chromosomes Ostrich chromosomes Saker falcon chromosomes
  No. PCFs placed 46 53 64
  No. PCFs oriented 28 37 37
  Disagreements RACA-FISH 4 0 0
  Length placed (bp) 1,013,720,408 969,537,146 1,055,312,481
  Length oriented (bp) 844,433,024 869,521,333 790,725,803
  % original assembly placed 93.56 79.45 90.12
  % original assembly oriented 77.93 71.26 67.52