Skip to main content

Advertisement

Table 1 Statistics for the scaffold split regions tested by PCR

From: Chromosome-level assembly reveals extensive rearrangement in saker falcon and budgerigar, but not ostrich, genomes

Statistics Saker falcon Ostrich Budgerigar
Pair-end read physical coverage within tested scaffolds 135–524 2–604 0–631
No. split SF adjacencies by RACA (default param.) 61 69 154
No. tested scaffold split regions 22 (100%) 49 (100%) 43 (100%)
No. amplified split regions (confirmed SF joints) 11 (50%) 32 (65%) 20 (46%)
No. non-amplified split regions 11 (50%) 17 (35%) 23 (54%)
No. tested RACA-suggested adjacencies 11 8 18
No. amplified adjacencies (chimeric SF joints) 5 7 11
Final no. ambiguous SF joints from tested split regions 6 10 12
Selected pair-end read spanning threshold 379 239 216