Fig. 4

Coordinated mRNA and protein covariation in differentiating ES cells. a Clustergram of pairwise correlations between mRNAs with 2.5 or more reads per cell as quantified by inDrop in single EB cells [47]. b Clustergram of pairwise correlations between proteins quantified by SCoPE-MS in 12 or more single EB cells. c The overlap between corresponding RNA from a and protein clusters from b indicates similar clustering patterns. d Protein-protein correlations correlate to their corresponding mRNA-mRNA correlations. Only genes with significant mRNA-mRNA correlations were used for this analysis. e The concordance between corresponding mRNA and protein correlations (computed as the correlation between corresponding correlations [42]) is high for ribosomal proteins (RPL and RPS) and lower for developmental genes; distribution medians are marked with red pluses. Only the subset of genes quantified at both RNA and protein levels were used for all panels