Fig. 3

Performance comparison between PHLI-seq and commercially available laser microdissection techniques. LMD technique uses UV laser to dissect a region of a sample and the polymer membrane which supports the region. LPC technique also uses UV laser but catapults a region or a cell by pressure generated by laser-produced plasma. a We measured amplification start time by real-time monitoring of the MDA reactions to measure the quality of the nucleic acid in the isolated cells. b Also, we measured the time from targeting the cells at the computer interface to when the cells were completely isolated. These results showed that PHLI-seq outperforms commercial laser microdissection in terms of quality of DNA in isolated cells and process speed. c Using whole-genome sequencing data, each sample was evaluated by genome alignment ratio and copy number correlation to HL60 genome. d, e Lorenz curve provided an insight on the DNA quality after isolating cells by PHLI-seq, LPC, and LMD. This result showed that DNA quality was better when cells were isolated by PHLI-seq than LMD or LPC. f CNA plots of isolated single cells demonstrated that PHLI-seq could provide uniformly amplified genome with correct copy number, whereas large deletions or severe amplification bias were observed in LPC or LMD