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Fig. 6 | Genome Biology

Fig. 6

From: CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters

Fig. 6

On-target modulation frequency distribution of gRNAs that are binned into low, medium, and high specificity groups using CRISPRspec method. Distributions are given as kernel density estimates (filled curves) together with the cumulative distribution function (dashed lines) of on-target modulation frequencies for each specificity group, separately for each dataset. Given modulation frequencies represents the cleavage efficiency of the intended on-target and are dataset specific. Triangles on the x-axis indicates the median values

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