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Fig. 2 | Genome Biology

Fig. 2

From: CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters

Fig. 2

Receiver operating characteristic (ROC) analysis of off-target scoring methods when benchmarked with the Haeussler dataset [11], allowing up to six mismatches, and NGG, NAG, and NGA PAM sequences for off-targeting. ROC curves for CFD and Elevation methods largely overlap and CRISPRoff shows the best performance with the largest area under its ROC curve. FPR and TPR values of the methods at specific points, indicated by dashed lines, are given in Table 1

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