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Fig. 6 | Genome Biology

Fig. 6

From: Dynamics and functional interplay of histone lysine butyrylation, crotonylation, and acetylation in rice under starvation and submergence

Fig. 6

Rice SRT2 has a decrotonylase activity. a Histone H3K9ac, Kbu, and Kcr levels in SRT1 RNAi, srt2 CRISPR, and hda705 CRISPR plants compared to wild types (MH63, DJ, and ZH11) were detected by immunoblotting. Each sample was loaded twice and two replicates for each immunoblot are shown. Relative quantified signals of each band are indicated with the first wild type loading set as 1. b In vitro lysine decrotonylation activity of OsSRT2 by fluorometric assays. Left panel, assays with E. coli-produced OsSRT1-GST protein. GST and mutated OsSRT2 (S123 Y and H215 Y in the catalytic domain) were used as control. Right panel: assays with OsSRT1 protein produced in transient transfected tobacco cells. Bars are means ± SD from three biological replicates

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Genome Biology

ISSN: 1474-760X

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