Table 1 Summary of the CRISPR-induced exon skipping in rabbits
From: CRISPR-induced exon skipping is dependent on premature termination codon mutations
Mutation type | Rabbits lines | Nature of mutation | Predicted ESS | PTC in exon | Exon skipping | Method |
|---|---|---|---|---|---|---|
Non-frameshift | D3 (Additional file 2: Figure S1) | − 75 bp in exon 51 | Yes | No | No | CRISPR/Cas9 |
L2 (Additional file 2: Figure S2) | (− 4, + 1) bp in exon 2 | Yes | No | No | CRISPR/Cas9 | |
K2 (Additional file 2: Figure S6) | WT/− 6 bp in exon 3 | Yes | No | No | CRISPR/Cas9 | |
K3 (Additional file 2: Figure S6) | − 6 bp in exon 3 | Yes | No | No | CRISPR/Cas9 | |
Missense | T2 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 |
T3 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
T4 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
T5 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
T6 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
PTCs | D2 (Additional file 2: Figure S1) | − 157 bp/− 70 bp in exon 51 | Yes | Exon 51 | Exon 51 (233 bp) | CRISPR/Cas9 |
L3 (Additional file 2: Figure S2) | − 106 bp/− 14 bp in exon 2 | Yes | Exon 2 | Exon 2 (157 bp) | CRISPR/Cas9 | |
A2 (Additional file 2: Figure S3) | − 10 bp/− 13 bp in exon 12 | Yes | Exon 12 | Exon 12 (223 bp) | CRISPR/Cas9 | |
G1 (Additional file 2: Figure S4) | − 91 bp in exon 5 | Yes | Exon 5 | Exon 5 (130 bp) | CRISPR/Cas9 | |
G2 (Additional file 2: Figure S4) | − 14 bp in exon 5 | Yes | Exon 5 | Exon 5 (130 bp) | CRISPR/Cas9 | |
G3 (Additional file 2: Figure S4) | − 5 bp/− 91 bp in exon 5 | Yes | Exon 5 | Exon 5 (130 bp) | CRISPR/Cas9 | |
G4 (Additional file 2: Figure S4) | − 5 bp in exon 5 | Yes | Exon 5 | Exon 5(130 bp) | CRISPR/Cas9 | |
B3 (Additional file 2: Figure S5) | C>T in exon 20 | Yes | Exon 20 | Exon 20 (242 bp) | BE3 | |
B4 (Additional file 2: Figure S5) | C>T in exon 20 | Yes | Exon 20 | Exon 20 (242 bp) | BE3 | |
PTCs in exon 1 | M2 (Additional file 2: Figure S8) | C>T in exon 1 | No | Exon 1 | No | BE3 |
M3 (Additional file 2: Figure S8) | C>Tin exon 1 | No | Exon 1 | No | BE3 | |
Y2 (Additional file 2: Figure S9) | C>T in exon 1 | No | Exon 1 | No | BE3 | |
Y3 (Additional file 2: Figure S9) | C>T in exon 1 | No | Exon 1 | No | BE3 |