Skip to main content

Table 1 Summary of the CRISPR-induced exon skipping in rabbits

From: CRISPR-induced exon skipping is dependent on premature termination codon mutations

Mutation type Rabbits lines Nature of mutation Predicted ESS PTC in exon Exon skipping Method
Non-frameshift D3 (Additional file 2: Figure S1) − 75 bp in exon 51 Yes No No CRISPR/Cas9
L2 (Additional file 2: Figure S2) (− 4, + 1) bp in exon 2 Yes No No CRISPR/Cas9
K2 (Additional file 2: Figure S6) WT/− 6 bp in exon 3 Yes No No CRISPR/Cas9
K3 (Additional file 2: Figure S6) − 6 bp in exon 3 Yes No No CRISPR/Cas9
Missense T2 (Additional file 2: Figure S7) C>T in exon 14 No No No BE3
T3 (Additional file 2: Figure S7) C>T in exon 14 No No No BE3
T4 (Additional file 2: Figure S7) C>T in exon 14 No No No BE3
T5 (Additional file 2: Figure S7) C>T in exon 14 No No No BE3
T6 (Additional file 2: Figure S7) C>T in exon 14 No No No BE3
PTCs D2 (Additional file 2: Figure S1) − 157 bp/− 70 bp in exon 51 Yes Exon 51 Exon 51 (233 bp) CRISPR/Cas9
L3 (Additional file 2: Figure S2) − 106 bp/− 14 bp in exon 2 Yes Exon 2 Exon 2 (157 bp) CRISPR/Cas9
A2 (Additional file 2: Figure S3) − 10 bp/− 13 bp in exon 12 Yes Exon 12 Exon 12 (223 bp) CRISPR/Cas9
G1 (Additional file 2: Figure S4) − 91 bp in exon 5 Yes Exon 5 Exon 5 (130 bp) CRISPR/Cas9
G2 (Additional file 2: Figure S4) − 14 bp in exon 5 Yes Exon 5 Exon 5 (130 bp) CRISPR/Cas9
G3 (Additional file 2: Figure S4) − 5 bp/− 91 bp in exon 5 Yes Exon 5 Exon 5 (130 bp) CRISPR/Cas9
G4 (Additional file 2: Figure S4) − 5 bp in exon 5 Yes Exon 5 Exon 5(130 bp) CRISPR/Cas9
B3 (Additional file 2: Figure S5) C>T in exon 20 Yes Exon 20 Exon 20 (242 bp) BE3
B4 (Additional file 2: Figure S5) C>T in exon 20 Yes Exon 20 Exon 20 (242 bp) BE3
PTCs in exon 1 M2 (Additional file 2: Figure S8) C>T in exon 1 No Exon 1 No BE3
M3 (Additional file 2: Figure S8) C>Tin exon 1 No Exon 1 No BE3
Y2 (Additional file 2: Figure S9) C>T in exon 1 No Exon 1 No BE3
Y3 (Additional file 2: Figure S9) C>T in exon 1 No Exon 1 No BE3
  1. The ESS sites were predicted by online tool (http://astlab.tau.ac.il/index.php)
  2. −, deletion; +, insert; BE3, Cytidine base editors; ESS, essential splice site
  3. D1, D2, D3, the DMD gene-edited rabbits by CRISPR/Cas9; L1, L2, L3, the LMNA gene-edited rabbits by CRISPR/Cas9; K1, K2, K3, the GCK gene-edited rabbits by CRISPR/Cas9; A1, A2, the ANO5 gene-edited rabbits by CRISPR/Cas9; G1, G2, G3, G4, G5, the GHR gene-edited rabbits by CRISPR/Cas9; B1, B2, B3, B4, the DMD gene-edited rabbits by BE3; T1, T2, T3, T4, T5, T6, the TIA1 gene-edited rabbits by BE3; M1, M2, M3, the MSTN gene-edited rabbits by BE3; Y1, Y2, Y3, the TYR gene-edited rabbits by BE3