Fig. 7

Analysis of the rs12144978-associated chromatin loops. Guide RNAs targeting regions encompassing CTCF site 1 (the PCa risk-associated CTCF site), CTCF site 2, and/or CTCF site 3 (or the empty guide RNA vector as a control) were introduced into 22Rv1 prostate cancer cells, along with Cas9. Cell pools were harvested, and KCNN3 expression was analyzed by RT-qPCR. Shown within the blue bars is the fold change in KCNN3 expression in the pools that received guide RNAs vs. the vector control. The yellow X indicates which CTCF site has been deleted; the size of each deletion can be found in Additional file 5: Table S4